Plant Science Making Use of 3D Laser Scanning

Image of Plant Science Advanced with 3D Laser Scanning

Plant Science Advanced with 3D Laser Scanning

Father of genetics and plant science Gregor Mendel spent years tediously observing and measuring pea plant traits by hand in the 1800s to uncover the basics of genetic inheritance. Today, botanists can track the traits, or phenotypes, of hundreds or thousands of plants much more quickly, with automated camera systems. Now, Salk researchers have helped speed up plant phenotyping even more, with machine-learning algorithms that teach a computer system to analyze three-dimensional shapes of the branches and leaves of a plant. The study, published in Plant Physiology on October 7, 2019, may help scientists better quantify how plants respond to climate change, genetic mutations or other factors.

From an article in EurekaAlert!

“What we’ve done is develop a suite of tools that helps address some common phenotyping challenges,” says Saket Navlakha, an associate professor in Salk’s Integrative Biology Laboratory and Pioneer Fund Developmental Chair.

A plant’s environment helps dictate its structure, which is related to its health. Scientists trying to understand plant growth, engineer more resilient plants or boost crop production often want to measure detailed characteristics of a plant’s leaves and shoots. To do this phenotyping in a high-throughput way, many researchers use camera systems that take images of each plant from various angles and assemble a three-dimensional model. However, some measurements are hard to take with these stitched-together images.

Recently, some have turned to a new method, called 3D laser scanning, to capture the structure of plant architectures. Researchers shine a laser at each plant to “paint” its surface with the beam. The resulting data–called a 3D point cloud–portrays the fine detail of the plant’s surface. But quantitatively analyzing the point clouds can be challenging since the technology is so new and the datasets so large.

“The resolution and accuracy of this data is much higher,” says Navlakha. “But the methods that have been developed for analyzing leaves and branches in 2D images don’t work as well for these 3D point clouds.”

Navlakha, along with UC San Diego graduate student Illia Ziamtsov, used a 3D laser scanner to scan 54 tomato and tobacco plants grown in a variety of conditions. Then, they inputted the resulting 3D point clouds into machine-learning algorithms that let them teach the program how to phenotype the plants. The technique involved the researchers first indicating manually where leaves and shoots on the plants were. Then, the software began to automatically recognize these features.

“It’s like teaching things to a baby,” says Navlakha. “You give them examples of what a leaf looks like and what a branch looks like, and eventually they can identify a plant they’ve never seen before and pick out the leaves and branches.”

For the full article click here.

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